Flow Cytometry

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For More Information
Contact Evan Peterson: (801) 793-5242; evan.peterson@usu.edu or Chris Davies: (435) 797-1900; chris.davies@usu.edu

The Flow Cytometry group is a core facility of the Center for Integrated BioSystems at Utah State University. The facility provides instrumentation, personnel, and expertise to assist researchers in flow cytometry and fluorescence-activated cell sorting (FACS) applications.

The laboratory is equipped with a BD Biosciences Special Order FACSAria™ II, which is a high-speed FACS that can perform high-resolution, multicolor flow cytometry analysis. Our FACSAria™ II has 4 lasers that can detect up to 13 different colors in addition to the cell sorting functionality, and uses BD FACSDiva™ software for data acquisition and analysis.

For a complete list of available filter sets, lasers, and wavelengths, see our filter worksheet. Services are offered as fee for service (see fee schedule). For more information please contact Evan Peterson.

Analysis

  • The FACSAria™ is operated by a Flow Cytometry facility member.
  • Many applications on the equipment are possible; examples already run include transgenic cell line analysis, leukocyte phenotyping, plant ploidy analysis, and cell cycle analysis.
  • BD FACSDiva™ software is available for data acquisition and analysis in conjunction with applications.
  • FlowJo analysis software also available upon special request.

Sorting

  • Up to four different populations can be sorted simultaneously in tube format from a heterogeneous single sample.
  • The ACDU unit allows for sorting with plates or slides-compatible with 6, 24, 48, 96 and 384 well plates.
  • Additionally, the ACDU unit allows for sorting of single cells for cloning or potential single cell gene expression analysis using the BioMark™ (see Genotyping Core Lab).
  • Sorting options include semi-sterile (clean) or sterile (very clean), which requires longer set-up time resulting in increased cost.