Stephan Van Vliet: stephan.vanvliet@usu.edu
The Proteomics group is a core facility of the Center for Integrated BioSystems at Utah State University. It exists to help researchers with novel biomarker discovery, discovering the molecular basis for disease, small molecule extraction and identification from organic and aqueous mixtures.
Support equipment includes gel electrophoresis units, a GE Typhoon Laser Imager, an AB Sciex Triple Quad 7500 LC/MS-MS, and a GCT Premier mass spectrometer.
Available services include determination of melamine content, leachable/extractables, small molecule survey scans, and confirmation of single compound accurate mass. Our staff is dedicated to providing rapid, reliable, and reasonably priced services and support. For inquiries please contact Patti Champine.
Mass Spectrometry
The Proteomics group provides analytic services that involve mass spectrometric techniques including interpretation, design and optimization of experiments upon request.
Services include gas or liquid chromatography for small volume samples, molecule mass and chemical separation, identification and quantification (LC/MS or GC/MS).
The new AB Sciex Triple Quad 7500 LC/MS-MS that the CIB recently purchased is one of the most sensitive instruments currently available on the market. The instrument can be used to support a wide range of liquid-chromatography/mass-spectrometry applications, including metabolomics, lipidomics, or proteomics. The instrument is capable of doing both targeted and untargeted analysis. Please contact Stephan Van Vliet or Patti Champine on how we may support your analytical needs.
Protein Purification and Separation
The Proteomics group provides standard 1D or 2D protein separation, Western Blotting using our Bio-Rad 1- or 2-D Precast Gel Systems.
2-D difference gel electrophoresis (2D DIGE) using different fluorescent dyes to label control and treated samples, including an internal standard on each gel. This technology minimizes gel-to-gel variation and significantly increases accuracy and reproducibility. We can provide gel studies in three different sizes, 7cm, 11cm or 24cm. Analysis of DIGE gel images is also available.
Imaging
The Proteomics group provides platforms for imaging which include phosphorescent film, visible and fluorescent gels.
Equipment for gel imaging includes a Kodak 2000R Image Station for phosphorescent film and a GE Healthcare Typhoon Trio+ Laser Imager for UV and fluorescent gels.