Proteomics

Info
For More Information
Contact Patti Champine: (435) 797-7374; patti.champine@usu.edu

The Proteomics group is a core facility of the Center for Integrated BioSystems at Utah State University. It exists to help researchers with novel biomarker discovery, discovering molecular models for disease, small molecule extraction and identification from organic and aqueous mixtures.

Support equipment includes gel electrophoresis units, a GE Typhoon Laser Imager, a Waters nanoACQUITY Ultra Performance LC™ (UPLC™) System, and a GCT Premier mass spectrometer.

Available services include determination of melamine content, leachable/extractables, small molecule survey scans, and confirmation of single compound accurate mass. Our staff is dedicated to providing rapid, reliable, and reasonably priced services and support. For inquiries please contact Patti Champine.

Mass Spectrometry

Mass Spectrometry

The Proteomics group provides analytic services that involve mass spectrometric techniques including interpretation, design and optimization of experiments upon request.

Services include liquid chromatography for small volume samples, molecule mass and chemical separation, identification and quantification (GC/MS).

Support equipment includes a Waters GCT Premier Mass Spectrometer, and a Waters NanoAcquity Ultra Performance LC™ (UPLC™) System.

Protein Purification and Separation

The Proteomics group provides standard 1D or 2D protein separation, Western Blotting using our Bio-Rad 1- or 2-D Precast Gel Systems.

2-D difference gel electrophoresis (2D DIGE) using different fluorescent dyes to label control and treated samples, including an internal standard on each gel. This technology minimizes gel-to-gel variation and significantly increases accuracy and reproducibility. We can provide gel studies in three different sizes, 7cm, 11cm or 24cm. Analysis of DIGE gel images is also available.
Mass Spectrometry
Mass Spectrometry

Imaging

The Proteomics group provides platforms for imaging which include phosphorescent film, visible and fluorescent gels.

Equipment for gel imaging includes a Kodak 2000R Image Station for phosphorescent film and a GE Healthcare Typhoon Trio+ Laser Imager for UV and fluorescent gels.